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Mutate for Digest

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atgtctgattcgctaaatcatccatcgagttctacggtgcatgcagatgatggattcgag
ccaccaacatctccggaagacaacaacaaaaaaccgtctttagaacaaattaaacaggaa
agagaagcgttgtttacggatctattcgcagatcgtcgacgaagcgctcgttctgtgatt
gaagaagctttccaaaacgaactcatgagtgctgaaccagtccagccaaacgtgccgaat
ccacattcgattcccattcgtttccgtcatcaaccagttgctggacctgctcatgatgtt
ttcggagacgcggtgcattcaatttttcaaaaaataatgtccagaggagtgaacgcggat
tatagtcattggatgtcatattggatcgcgttgggaatcgacaaaaaaacacaaatgaac
tatcatatgaaaccgttttgcaaagatacttatgcaactgaaggctccttagaagcgaaa
caaacatttactgataaaatcaggtcagctgttgaggaaattatctggaagtccgctgaa
tattgtgatattcttagcgagaagtggacaggaattcatgtgtcggccgaccaactgaaa
ggtcaaagaaataagcaagaagatcgttttgtggcttatccaaatggacaatacatgaat
cgtggacagagtgacatttcacttcttgcggtgttcgatgggcatggcggacacgagtgc
tctcaatatgcagctgctcatttctgggaagcatggtccgatgctcaacatcatcattca
caagatatgaaacttgacgaactcctagaaaaggctctagaaacattggacgaaagaatg
acagtcagaagtgttcgagaatcttggaaaggtggaaccactgctgtctgctgtgctgtt
gatttgaacactaatcaaatcgcatttgcctggcttggagattcaccaggttacatcatg
tcaaacttggagttccgcaaattcactactgaacactccccgtctgacccggaggaatgt
cgacgagtcgaagaagtcggtggccagatttttgtgatcggtggtgagctccgtgtgaat
ggagtactcaacctgacgcgagcactaggagacgtacctggaagaccaatgatatccaac
aaacctgataccttactgaagacgatcgaacctgcggattatcttgttttgttggcctgt
gacgggatttctgacgtcttcaacactagtgatttgtacaatttggttcaggcttttgtc
aatgaatatgacgtagaagattatcacgaacttgcacgctacatttgcaatcaagcagtt
tcagctggaagtgctgacaatgtgacagtagttataggtttcctccgtccaccagaagac
gtttggcgtgtaatgaaaacagactcggatgatgaagagagcgagctcgaggaagaagat
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